Gerhard Struber became convinced after Saturday’s win at QPR that goalkeeper Sami Radlinger and midfielder Kenny Dougall had ‘no future’ at Oakwell.
The Reds had tried to agree short-term deals with both players but they have now been released.
Their contracts are due to expire on Tuesday and Struber says they will not play against Millwall or Blackburn Rovers in the coming days.
The 27-year-olds were both on the bench on Saturday, with Dougall coming on. But youngsters Jack Walton and Romal Palmer impressed in their positions.
Struber told the Chronicle: “We were in a very good exchange and my opinion was that they go with me until the last game but, it is clear for me after the last game, they have no future in Barnsley.
“I have spoken to them very openly. Kenny had a long time in Barnsley but the gap between my style of play and the DNA of Kenny is too big to go together in the future. I hope they find a good challenge. They will train until the end of June but they have no chance of gametime.”
He added: “It is not easy for the team but it’s OK. We have many players in a good shape and many players with the right attitude.”
Dani Pinillos, Mamadou Thiam and Jared Bird have also left.