Wombwell Main, based on Windmill Road, closed yesterday.
Club secretary Craig Bond stressed the health of staff and the local community was paramount in their decision.
He said: “Wombwell Main took the decision to close the club for the weekend after a member of staff tested positive for Covid-19.
“All activities such as football and cricket are suspended at the club.
“Closing the club is our decision for health and safety reasons and for the good of the community we are part of.
“We have been in touch with licensing and they have commended all our actions so far.
“Track and trace have also been in touch, and the association, and we have followed all protocols required.
“All staff members have been tested and have all come back with negative results.
“We will be assessing the situation on a day-to-day basis and the club will reopen when it’s safe to do so.”