Kyle Swann, Harvey Kennett and Curtis Horbury were attending a match between Southend United and the Reds at Roots Hall Stadium on March 2, 2019.
Widely-circulated video footage recorded during the game - which Barnsley won 3-0 - shows stewards attempting to deal with an incident within the crowd.
The stewards were assaulted by Swann, 19, of Laithes Lane, Athersley, Kennett, 19, of Newstead Road, Athersley and Horbury, 21, of Station Road, Barnsley.
All three men were identified following Essex Police’s public appeal before being charged.
Darren Kennett, 54, of Newstead Road, Athersley, Cameron Rose, 23, of Little Stubbing, Wombwell, Jonathan Senior, 47, of Calabria Grove, Worsbrough Common and Tom Bracken, 35, of Oakley Avenue, West Melton, deny affray.
All four men will next appear at Basildon Crown Court next Friday to set a trial date.