Vandals hit The Snap Tin cafe on Barnburgh Lane, Goldthorpe, last Thursday night and left behind broken windows and graffiti.
It came days after the previous incident in which the cafe’s scaffolding was stolen during another overnight raid.
The cafe was due to unveil its new extension which will help to host their community classes, but this has now been halted while repairs are carried out.
Now Anita Heatherglen, the owner, is keen to find those responsible for the destruction.
She told the Chronicle: “We were really looking forward to get up and running and move into our new building.
“We had only just fitted the front door earlier that day before it was smashed. Now it’s completely destroyed.
“This is a massive financial setback for us - just as we were getting back on our feet.
“It’s a kick in the teeth at a time when we should be pulling together. I can’t thank the community enough for their tireless support and we hope to be in the new building again soon.”
A spokesperson for South Yorkshire Police added: “Police are investigating reports of criminal damage at a Goldthorpe business.
”At around 6.30pm on November 12, it is reported that persons unknown caused damage to a building on Barnburgh Lane.
”It is understood that doors and windows have been damaged and smashed. Enquiries are ongoing.”