The new two-storey, three-bay fire station is housed on Broadway and replaces the 60-year-old former building on the same site which had reached the end of its operational life.
The energy efficient building was completed earlier this month and finally became operational on Tuesday.
Fire crews from the station had been housed in a temporary off-site facility whilst the construction was being completed.
Assistant chief fire officer Tony Carlin said: “This is a really special day, as the new station has been several years in the planning and represents part of our vision to provide all our firefighters with modern, comfortable and energy efficient spaces in which to live and work.
“The new station has been completed to a very high specification and with the wishes of the firefighters who work there taken into account at every stage.
“It has been paid for using money set aside from the authority’s reserves and we expect it to provide an outstanding service to the people of Barnsley for many years to come.”