This week, the charity have been parked up outside Locke Park in a £250,000 bus funded by the Postcode Lottery.
From Wednesday to Friday, free checks were provided to any pet on a drop-in basis.
A vaccination crew were on standby throughout the week to offer appointment-based treatments.
Vet Sue Bartlett, PDSA vet nurse, said: “People in Barnsley are very lucky as this is one of the first places in the UK to benefit from our new PetWise vehicle offering state-of-the-art facilities, so we can help pets and their owners.
“We know people love their pets and want the best for them, which is why PDSA is here to help. Thanks to support from the players of People’s Postcode Lottery we can offer free, friendly advice and support, helping owners to make small changes that often make big differences to the wellbeing of pets in Barnsley.”