The club, now dubbed The Comedy Shop, has been revived by entertainer Steve White and local businessman David Clayton.
The comedy club was founded in 1998 by stand-up act Toby Foster - and weekly shows featuring some of comedy’s greatest would perform in front of Barnsley crowds.
Now the new club will have its first permanent home in a dedicated venue on Graham’s Orchard, just off Shambles Street and adjacent to the Soho Music Institute.
Steve said: “I’ve been a big supporter of Barnsley for many years and I’m a huge fan of the entertainment the town already has on offer.
“This is a chance for us to bring something extra to Barnsley by providing top name comedy every week for people to enjoy whilst then also experiencing the incredible restaurants and bars that surround us.
“I’m really excited about the comedians we already have booked.”
Some of the names already booked to appear are Paul Tonkinson, Tom Wrigglesworth and Matt Reed.
The venue will open on Friday, April 8 and tickets can be purchased on wegottickets.com by searching The Comedy Shop, Barnsley.