Police were forced to cordon off a number of streets following the incident on New Winterwell Road at around 3.40am.
A man, with no fixed address, and in his thirties was taken to hospital by an ambulance crew with what are believed to be serious injuries, in line with stab wounds.
A police spokesperson said: “Officers attended the scene and made two arrests a 24-year-old woman and a 50-year-old man, both from Rotherham - on suspicion of Section 18 wounding.
“Both remain in police custody as enquiries continue.
“Detectives believe this to be an isolated incident where those involved were known to each other.
“If you have any information which could assist our officers, please contact 101 quoting incident number 99 of April 12.”